Xiphophorus maculatus Genome Assembly

WashU Genome Assembly version 4.4.2; JHP Genetic Map Assembly 1.0

Angel Amores, Julian Catchen, Indrajit Nanda, Wesley Warren, Ron Walter, Manfred Schartl, and John Postlethwait. A RAD-tag Genetic Map for the Platyfish (Xiphophorus maculatus) Reveals Mechanisms of Karyotype Evolution Among Teleost Fish. Genetics, 197:2, 625-641. [reprint]

Mapped sequence: 653,641,933bp; Unmapped sequence: 71,161,821bp.

Retrieve the FASTA sequence: xma_washu_4.4.2-jhp_1.0.fa
Unplaced scaffolds: xma_washu_4.4.2-jhp_1.0.unplaced_scaffolds.fa
Genome AGP definition for scaffolds: xma_washu_4.4.2-jhp_1.0.agp
Genome AGP definition for chromosomes: xma_washu_4.4.2-jhp_1.0.flat.agp

View the assembly logs:

LG1 LG5 LG9 LG13 LG17 LG21
LG2 LG6 LG10 LG14 LG18 LG22
LG3 LG7 LG11 LG15 LG19 LG23
LG4 LG8 LG12 LG16 LG20 LG24

Assembly Statistics:

15292 total markers mapped; 770 conflicting markers excluded.
102 scaffolds mapped to multiple linkage groups.
212 scaffolds were split:
  2 fragments: 139 cases
  3 fragments: 43 cases
  4 fragments: 22 cases
  5 fragments: 5 cases
  6 fragments: 2 cases
  7 fragments: 1 cases
2271 total scaffolds mapped from 1944 original scaffolds. 653,417,233bp total length.

Problem Scaffolds

These scaffolds appear to be misassembled, however markers were in conflict when a split was attempted.

Scaffolds split across linkage groups Scaffolds split within a linkage group
JH556770.1
JH556689.1
JH557978.1
JH557060.1
JH556666.1 [markers overlap]
JH556815.1 [markers overlap]
JH556723.1 [too many markers at node 207]
JH556705.1 [markers overlap]
JH556673.1 [markers overlap]
JH556672.1 [markers overlap]
JH558760.1 [too many 'start' or 'end' markers at node 0]
JH556715.1 [markers overlap]
JH556938.1 [too many 'start' or 'end' markers at node 149]
JHP00225.0 [markers overlap]
JH556875.1 [markers overlap]
JHP00066.0 [markers overlap]

Gene Models

Genes were downloaded from Ensembl release 76 and their coordinates were translated to this chromosome assembly.

Processed 20,742 genes; 
  20,660 translated
  82 failures (26 on multiple chromosomes).
  37 genes on 1 mising scaffold (NC_011379)
Processed 520,477 GTF components:
  493,546 translations
  122 translation errors
  26,701 components on unassembled scaffolds (no translation necessary) 
108 components in 1 missing scaffolds.